1. Experiments
  2. ChIP-seq
  3. Drosophila melanogaster
  4. whole organism

Experiment summary for ENCSR303EBD

doi:10.17989/ENCSR303EBD

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Lpt
Biosample summary
Drosophila melanogaster strain Lpt-GFP whole organism prepupa at white prepupa stage genetically modified (insertion) using site-specific recombination targeting D. melanogaster Lpt
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Lpt-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Lpt-GFP
Date submitted
October 22, 2015
Date released
July 6, 2016
Submitter comment
ENCFF869ZVG, ENCFF205RVF were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain Lpt-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster LptENCBS461IYMENCGM116EIBENCAB728YTOENCLB474XIU
21Drosophila melanogaster strain Lpt-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster LptENCBS925HZCENCGM116EIBENCAB728YTOENCLB907UXU
11Drosophila melanogaster strain Lpt-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster LptENCBS392BRQENCGM116EIBENCAB728YTOENCLB918IYY

Files

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Output type
Replicates

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