Experiment summary for ENCSR453UOF
doi:10.17989/ENCSR453UOF
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- Pif1B
- Biosample summary
- Drosophila melanogaster strain Pif1B-GFP whole organism pupa genetically modified (insertion) using site-specific recombination targeting D. melanogaster Pif1B
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing Pif1B-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:Pif1B-GFP
- Date submitted
- July 24, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF228WWI, ENCFF441TZG, ENCFF692QRX, ENCFF916DYJ were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 3 | 1 | Drosophila melanogaster strain Pif1B-GFP mixed sex pupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Pif1B | ENCBS794JLC | ENCGM041CFD | ENCAB728YTO | ENCLB607HNQ |
| 1 | 1 | Drosophila melanogaster strain Pif1B-GFP mixed sex pupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Pif1B | ENCBS915TDC | ENCGM041CFD | ENCAB728YTO | ENCLB468WZW |
| 2 | 1 | Drosophila melanogaster strain Pif1B-GFP mixed sex pupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Pif1B | ENCBS897XAJ | ENCGM041CFD | ENCAB728YTO | ENCLB157IUL |