1. Antibodies
  2. Homo sapiens
  3. FOXA2

ENCAB000AGO

Antibody against Homo sapiens FOXA2

Homo sapiens
liver
characterized to standards with exemption
Homo sapiens
HepG2
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-6554
Lot ID
L1409
Characterized targets
FOXA2 (Homo sapiens)
Host
goat
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope mapping at the N-terminus of FOXA2 of human origin

Characterizations

FOXA2 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target FOXA2 is represented by the attached position weight matrix (PWM) derived from files ENCFF478RQU and ENCFF967WGK. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations. For more information on the underlying statistical methods, please see the attached document. From ENCFF478RQU: Accept probability score: 0.83082051306, Global enrichment Z-score: 6.5416041216, Positional bias Z-score: 10.3040374013, Peak rank bias Z-score: 9.8421803755. From ENCFF967WGK: Accept probability score: 0.83082051306, Global enrichment Z-score: 6.95433992802, Positional bias Z-score: 10.9149340941, Peak rank bias Z-score: 9.38129982694, Enrichment rank: 1.0.
Submitted by
Aditi Narayanan
Lab
Richard Myers, HAIB
Download
 ENCAB000AGO.pdf
FOXA2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Whole cell lysates of HepG2 were immunoprecipitated using the primary antibody (Santa Cruz Biotechnology; sc-6554). The IP fraction was separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. The membrane was probed with the primary antibody (same as that used for IP) and a secondary HRP-conjugated antibody. The resulting bands were visualized with SuperSignal West Femto Solution (Thermo Scientific). Protein Marker (PM) is labeled in kDa. The approximate size of FOXA2 is ~54 kDa.
Reviewer comment
Band is not visible
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB
FOXA2 (Homo sapiens)
liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
The ENCODE Binding Working Group finds for some valuable tissues that recreating a primary on well characterized antibodies is not cost effective. Therefore, they allow exemption from standards for these tissues.
Submitter comment
The lab is asking for an exemption for liver cells due to the lack of resource to make a primary characterization for them
Reviewer comment
Exempted by the Feb 29, 2016 antibody review panel
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
Download
 No_tissue.png