1. Antibodies
  2. Homo sapiens
  3. TBL1XR1

ENCAB000ALP

Antibody against Homo sapiens TBL1XR1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Homo sapiens
K562, HeLa-S3, HepG2
partially characterized
Homo sapiens
GM12878
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab24550
Lot ID
GR340121
Characterized targets
TBL1XR1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
Immunogen: Recombinant full length protein (Human).

Characterizations

TBL1XR1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Supporting characterizations for a different lot of this antibody was submitted under ENCAB596WIK in K562 and HepG2 for comparison as allowed by section b on page 4 of the May 2016 ENCODE TF Antibody Characterization Standards Document
Submitter comment
We ran out of this lot and could not use it in further characterizations. We have used a different lot of the same antibody (ENCAB596WIK) to demonstrate that a similar binding pattern is achieved in K562 as in this lot, as allowed by section b on page 4 of the May 2016 ENCODE TF Antibody Characterization Standards document.
Reviewer comment
Although section b on page 4 of the May 2016 ENCODE TF Antibody Characterization Standards document requires the previously characterized antibody of a different lot number to be fully characterized (i.e. have both compliant primary and secondary characterizations), this requirement was waived by ENCODE Antibody Review Panel for this lot on August 23, 2017.
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
TBL1XR1 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
not reviewed
Caption
Using antibody ab24550 or NB600-270, we observe a single band in Western blots on lysates from cell lines K562 and HeLa S3, that migrates at a position consistent with the predicted size (~56kD) of TBLR1 (Panels A,C). This band is specifically and efficiently immunoprecipitated from K562 lysates by each antibody (Panels B,D). Based on this, ab24550 and NB600-270 are validated by this criterion.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
TBL1XR1 (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
Because the overlap of the top 40% of peaks from datasets derived from antibodies NB600-270 and ab24550 (compared using standard ENCODE scoring methods) exceeds the recommended threshold of 80%, both antibodies meet this criterion for validation.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
TBL1XR1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
B. Immunoprecipitation was performed on nuclear lysates from K562 cells using antibody ab24550. Lane1: Nuclear lysate. Lane 2: Unbound material from immunoprecipitation with ab24550. Lane 3: Bound material from immunoprecipitation with ab24550. Lane 4: Bound material from control immunoprecipitation with rabbit IgG. Arrow indicates band of expected size (56kD) that is highly enriched in the specifically immunoprecipitated fraction.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
TBL1XR1 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
A. Western blot using antibody ab24550 on nuclear lysates from cell lines GM12878 (Lane1), K562 (Lane2), HeLaS3 (Lane3), and HepG2 (Lane4). Expected band size is 56 kD. Arrow indicates band of expected size (56 kD) that is highly enriched in the specifically immunoprecipitated fraction.
Reviewer comment
Esther Chan: lanes 1 and 4 are not compliant because there is no signal
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford