ENCAB000APU

Alternate accession: ENCAB000ANW

Antibody against Homo sapiens H3K9me3, Mus musculus H3K9me3

Bos taurus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards with exemption
Status
released
Source (vendor)
Abcam
Product ID
ab8898
Lot ID
484088
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, tri methylated at K9.
Aliases
bradley-bernstein:PchAb 122-V

Characterizations

H3K9me3 (Homo sapiens)
thymusthymus
Method: immunoblot
compliant
Submitter comment
This western blot demonstrates the antibody recognizes a protein of the molecular weight consistent with human histone H3
Reviewer comment
Though this western blot was carried out with calf biosamples, the ENCODE Antibody Review Panel has reviewed this characterization and allowed this lot to be exempted for use in human biosamples on November 11, 2016.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K9me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K9me3 antibody was the correct one.
Submitted by
Meital Hatan
Lab
Bradley Bernstein, Broad
H3K9me3 (Mus musculus)
Method: dot blot assay
compliant
Submitted by
Charles Epstein
Lab
Bradley Bernstein, Broad