1. Antibodies
  2. Homo sapiens
  3. CIRBP

ENCAB000BFF

Antibody against Homo sapiens CIRBP

Homo sapiens
K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN032P
Lot ID
001
Characterized targets
CIRBP (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
KLH conjugated synthetic peptide, corresponding to N-terminus of human CIRBP
External resources

Characterizations

CIRBP (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against CIRBP in HepG2 whole cell lysate using CIRBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CIRBP. CIRBP protein appears as the green band, Beta-actin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
CIRBP (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against CIRBP in K562 whole cell lysate using CIRBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CIRBP. CIRBP protein appears as the green band, Beta-actin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
CIRBP (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB characterization of CIRBP specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-CIRBP(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
CIRBP (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using CIRBP specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CIRBP antibody (lanes under 'CIRBP').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD