1. Antibodies
  2. Homo sapiens
  3. FXR2

ENCAB069PGU

Antibody against Homo sapiens FXR2

Homo sapiens
K562
characterized to standards
Homo sapiens
HepG2
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-894A
Lot ID
1
Characterized targets
FXR2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

FXR2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB characterization of FXR2 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal FXR2 Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
FXR2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against FXR2 in HepG2 whole cell lysate using FXR2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FXR2. FXR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
FXR2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against FXR2 in K562 whole cell lysate using FXR2 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FXR2. FXR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
FXR2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using FXR2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FXR2 antibody (lanes under 'FXR2').
Submitter comment
The placement of the arrow is incorrect, should be around the 85-90kDa mark as the band is running a bit high in lane 4.
Reviewer comment
Major band runs a bit high but is consistent with IP in K562 and the size of the band in the K562 and HepG2 knockdowns, so will allow the size deviation.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
FXR2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using FXR2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FXR2 antibody (lanes under 'FXR2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD