1. Antibodies
  2. Homo sapiens
  3. PRPF4B

ENCAB208ZGL

Antibody against Homo sapiens PRPF4B

Homo sapiens
HepG2
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-665A
Lot ID
1
Characterized targets
PRPF4B (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-894
External resources

Characterizations

PRPF4B (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody A301-665A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 116.987.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PRPF4B (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A301-665A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
PRPF4B (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody A301-665A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
SNRNP200 involves ATP binding and Poly (A) RNA binding. Both HNRNPUL1 and PRPF4B have interactions with NTRK1 (87KD), http://thebiogrid.org/114416/summary/homo-sapiens/prpf4b.html and http://thebiogrid.org/116281/summary/homo-sapiens/hnrnpul1.html, NTRK1 is not in the same gel slice with HNRNPUL1 and PRPF4B. Both ILF3 and PRPF4B have interactions with ARRB2 (46KD), http://thebiogrid.org/114416/summary/homo-sapiens/prpf4b.html and http://thebiogrid.org/109822/summary/homo-sapiens/ilf3.html, ARRB2 is not in the same gel slice with ILF3 and PRPF4B. Both ILF3 and PRPF4B have interactions with ARRB2 (46KD), http://thebiogrid.org/114416/summary/homo-sapiens/prpf4b.html and http://thebiogrid.org/109822/summary/homo-sapiens/ilf3.html, ARRB2 is not in the same gel slice with ILF3 and PRPF4B. PRPF8 and PRPF4B have interaction, http://thebiogrid.org/114416/summary/homo-sapiens/prpf4b.html. BSCL2 mainly locates in Endoplasmic reticulum membrane, involves lipid metabolism.
Reviewer comment
Though ILF3 is ranked higher than PRPF4B but as noted by the submitters, they have interaction partners in common.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford