ENCAB211WTE
Antibody against Homo sapiens H3K36me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Diagenode
- Product ID
- pAb-192-050
- Lot ID
- A1847-001P
- Characterized targets
- H3K36me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 724
- External resources
Characterizations
H3K36me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K36me3 antibody was the correct one.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390
H3K36me3 (Homo sapiens)
HeLa-S3
Method: immunoblot
compliant
- Caption
- We performed a WB using 20ug of nuclear lysate from HeLa nuclear extract. Extract was resolved by electrophoresis on a 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 8 minutes, Blocked in Superblock for 1 hour, Antibodies diluted in Superblock imaged in ChemiDoc.
- Reviewer comment
- The characterization was completed for HeLa-S3 only as K562 was not subject to be tested by the H3K36me3 antibody in question, even though it is represented in panel A (but not panel B). However, this is a sufficient characterization for all human samples.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390