1. Antibodies
  2. Homo sapiens
  3. PPP1R10

ENCAB400PQW

Antibody against Homo sapiens PPP1R10

Homo sapiens
K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN067PW
Lot ID
001
Characterized targets
PPP1R10 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Peptide, internal of human PPP1R10
External resources

Characterizations

PPP1R10 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the PPP1R10 specific antibody, RN067PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the PPP1R10-specific antibody, RN067PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitter comment
Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
PPP1R10 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using PPP1R10 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-PPP1R10pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
PPP1R10 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against PPP1R10 in HepG2 whole cell lysate using PPP1R10 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PPP1R10. PPP1R10 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
PPP1R10 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against PPP1R10 in K562 whole cell lysate using PPP1R10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PPP1R10. PPP1R10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn