ENCAB426GGE
Antibody against Aequorea victoria eGFP
Drosophila melanogaster
whole organism
partially characterized
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab290
- Lot ID
- unknown
- Characterized targets
- eGFP (Aequorea victoria)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- crude
- Antigen description
- This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.
- External resources
Characterizations
eGFP (Aequorea victoria)
whole organism
Method: immunoblot
compliant
- Caption
- Western blot with Ab290 anti-GFP (1:1000) and Licor IR680LT (Goat anti-Rabbit,1:20000). Protein from 100 mg of whole flies was extracted in 750uL T-PER (Fisher). Lane1: ladder. Lane2: 5 uL W1118 (no GFP) whole protein extract. Lane3: 5uL BDSC#5629 (nuclear GFP) whole protein extract. The band is expected at 29 kDa.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U41HG007355
- Download
- Ab290.jpg
eGFP (Aequorea victoria)
whole organism
Method: immunofluorescence
compliant
- Caption
- Expression patterns for four transcription factors. The left column of images are the transcription expression pattern measured by in-situ hybridization on wild-type embryos as reported by the Berkeley Drosophila Genome Project (insitu.frutfly.org; PMIDs: 17645804, 12537577, 24359758). Right column are immunofluorescence patterns of GFP fused with each transcription factor. Anti-GFP (Ab290) and Alexa Fluor 488 Goat Anti-Rabbit were used for IF on the transgenic animals (ENCDO269BCO, ENCDO038HYA, ENCDO195AJO, ENCDO180UMR).
- Reviewer comment
- Note that according to the 2011 standards, immunofluorescence characterizations should be paired with a knockdown or knockout secondary characterization to confirm that the antibody is detecting the protein of interest.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U41HG007355
- Download
- Ab290_IF.jpg