ENCAB442TBN
Antibody against Homo sapiens LEF1
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-487A
- Lot ID
- 1
- Characterized targets
- LEF1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1039
- External resources
Characterizations
LEF1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-487A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight: 45 KD
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 2.jpg
LEF1 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target LEF1 is represented by the attached position weight matrix (PWM) derived from ENCFF043YZF. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.66775712244 Global enrichment Z-score: 6.21538428592 Positional bias Z-score: 8.86262064528 Peak rank bias Z-score: 7.55915676258 4 Enrichment rank: 1.0
- Submitted by
- Jessika Adrian
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996