1. Antibodies
  2. Homo sapiens
  3. ELAC2

ENCAB519QXK

Antibody against Homo sapiens ELAC2

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
PA5-78553
Lot ID
UK2881508
Characterized targets
ELAC2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Recombinant protein encompassing a sequence within the center region of human ELAC2. The exact sequence is proprietary.
External resources

Characterizations

ELAC2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against ELAC2 in HepG2 whole cell lysate using ELAC2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ELAC2. ELAC2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
ELAC2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against ELAC2 in K562 whole cell lysate using ELAC2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ELAC2. ELAC2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
ELAC2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the ELAC2 specific antibody, PA5-78553. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ELAC2-specific antibody, PA5-78553. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD