1. Antibodies
  2. Homo sapiens + Mus musculus
  3. HIST2H3A + HIST2H3D + HIST2H3C + Hist1h3d + Hist1h3b + Hist2h3c2 + Hist2h3c1 + Hist1h3e + Hist1h3f + Hist2h3b + Hist1h3c

ENCAB610CEF

Antibody against Homo sapiens H3K4me3, Mus musculus H3K4me3

Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
04-745
Lot ID
2197316
Host
rabbit
Purification
crude
Isotype
IgG
Antigen description
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Aliases
bing-ren:49.6
External resources

Characterizations

H3K4me3 (Mus musculus)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100% (noted with red #1).
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100% (noted with red #1).
Submitted by
Cricket Sloan
Lab
Bing Ren, UCSD
H3K4me3 (Mus musculus)
liverlungstomach
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
An immunoblot using whole cell extract of three tissue types in mouse and one human colorectal carcinoma cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me3 (Homo sapiens)
HCT116
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
An immunoblot using whole cell extract of three tissue types in mouse and one human colorectal carcinoma cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD