ENCAB635SXP
Antibody against Homo sapiens CTCF, Mus musculus CTCF
Homo sapiens
SK-N-SH, RWPE1
characterized to standards
Mus musculus
embryonic stem cell, Patski, spleen
characterized to standards
Homo sapiens
GM12878, CD4-positive naive resting alpha-beta T cell, activated CD8-positive, alpha-beta T cell, activated naive CD4-positive, alpha-beta T cell, activated CD4-positive, alpha-beta memory T cell, activated CD4-positive, alpha-beta T cell, activated CD8-positive, alpha-beta memory T cell, CD8-positive, alpha-beta memory T cell, MCF-7, activated B cell, B cell, CD8-positive, alpha-beta T cell, CD4-positive, alpha-beta memory T cell, AG04449, HEK293, BJ, AG04450, CD4-positive, alpha-beta T cell, common myeloid progenitor, CD34-positive, GM10248, MCF 10A, HEK293T, A549, IMR-90, endothelial cell of umbilical vein, HCT116, WTC11, embryonic stem cell, Patski, spleen, stimulated activated naive CD4-positive, alpha-beta T cell, stimulated activated CD4-positive, alpha-beta memory T cell, stimulated activated CD4-positive, alpha-beta T cell, stimulated activated CD8-positive, alpha-beta memory T cell, stimulated activated CD8-positive, alpha-beta T cell, naive thymus-derived CD4-positive, alpha-beta T cell, Caco-2, A673, Calu3, PC-3, OCI-LY7, Panc1, PC-9, T-cell, activated T-cell, mammary epithelial cell
characterized to standards with exemption
- Status
- released
- Source (vendor)
- ABclonal
- Product ID
- A1133
- Lot ID
- A1133
- Characterized targets
- CTCF (Homo sapiens), CTCF (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Recombinant protein of human CTCF
- External resources
Characterizations
CTCF (Homo sapiens)
GM12878CD4-positive naive resting alpha-beta T cellactivated CD8-positive, alpha-beta T cellactivated naive CD4-positive, alpha-beta T cellactivated CD4-positive, alpha-beta memory T cellactivated CD4-positive, alpha-beta T cellactivated CD8-positive, alpha-beta memory T cellCD8-positive, alpha-beta memory T cellMCF-7activated B cellB cellCD8-positive, alpha-beta T cellCD4-positive, alpha-beta memory T cellAG04449HEK293BJAG04450CD4-positive, alpha-beta T cellcommon myeloid progenitor, CD34-positiveGM10248MCF 10AHEK293TA549IMR-90endothelial cell of umbilical veinHCT116WTC11embryonic stem cellPatskispleenstimulated activated naive CD4-positive, alpha-beta T cellstimulated activated CD4-positive, alpha-beta memory T cellCD8-positive, alpha-beta memory T cellactivated CD4-positive, alpha-beta T cellstimulated activated CD4-positive, alpha-beta T cellstimulated activated CD8-positive, alpha-beta memory T cellstimulated activated CD8-positive, alpha-beta T cellnaive thymus-derived CD4-positive, alpha-beta T cellCaco-2A673Calu3PC-3OCI-LY7Panc1PC-9T-cellactivated T-cellmammary epithelial cell
Method: immunoblot
exempt from standards
- Caption
- Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types are exempted for this antibody by the ENCODE antibody review panel, because the antibody has satisfactory primary and secondary characterizations in two other cell types.
- Submitter comment
- This antibody was not characterized in other cell types due to lack of assays in the cell types with an approved CTCF antibody.
- Reviewer comment
- Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types are exempted for this antibody by the ENCODE antibody review panel.
- Submitted by
- Bonita Lam
- Lab
- Yijun Ruan, JAX
- Grant
- UM1HG009409
- Download
- AB_Exemption.png
CTCF (Mus musculus)
embryonic stem cellPatskispleen
Method: immunoblot
compliant
- Caption
- Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel 01.18.2022.
- Submitter comment
- This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
- Reviewer comment
- Approved by Erin Mendenhall 01.18.2022.
- Submitted by
- Khine Lin
- Lab
- Charles Lee
- Grant
- 3UM1HG009409-04S1
CTCF (Homo sapiens)
SK-N-SHRWPE1
Method: immunoprecipitation
compliant
- Submitter comment
- Approved by Eric Mendenhall from AB WG on 1/25/21.
- Submitted by
- Bonita Lam
- Lab
- Yijun Ruan, JAX
- Grant
- UM1HG009409
CTCF (Mus musculus)
Method: immunoprecipitation followed by mass spectrometry
exempt from standards
- Submitter comment
- This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
- Reviewer comment
- Approved by Erin Mendenhall 01.18.2022.
- Submitted by
- Khine Lin
- Lab
- Charles Lee
- Grant
- 3UM1HG009409-04S1
- Download
- exempted.png
CTCF (Homo sapiens)
Method: ChIP-seq comparison
compliant
- Caption
- Comparison of CTCF Abclonal antibody in ChIA-PET assays with compliant CTCF antibody from ChIP-seq antibody both performed on RWPE-1 cells
- Submitted by
- Khine Lin
- Lab
- Yijun Ruan, JAX
- Grant
- UM1HG009409
- Download
- RWPE1-qc.pdf
CTCF (Homo sapiens)
Method: ChIP-seq comparison
compliant
- Caption
- Comparison of CTCF Abclonal antibody in ChIA-PET assays with compliant CTCF antibody from ChIP-seq antibody both performed on SK-N-SH cells
- Submitted by
- Khine Lin
- Lab
- Yijun Ruan, JAX
- Grant
- UM1HG009409
- Download
- SK-N-SH-qc.pdf