ENCAB725WBH
Antibody against Homo sapiens POLR2A, Mus musculus POLR2A
Homo sapiens
K562, CD4-positive naive resting alpha-beta T cell, activated CD8-positive, alpha-beta T cell, CD8-positive naive resting alpha-beta T cell, GM12878, activated naive CD4-positive, alpha-beta T cell, activated CD4-positive, alpha-beta memory T cell, activated CD4-positive, alpha-beta T cell, activated naive CD8-positive, alpha-beta T cell, activated CD8-positive, alpha-beta memory T cell, CD8-positive, alpha-beta memory T cell, MCF-7, activated B cell, HepG2, RWPE1, B cell, CD8-positive, alpha-beta T cell, CD4-positive, alpha-beta memory T cell, AG04449, HEK293, BJ, AG04450, CD4-positive, alpha-beta T cell, common myeloid progenitor, CD34-positive, SK-N-SH, GM10248, MCF 10A, HEK293T, A549, endothelial cell of umbilical vein, HCT116, WTC11, embryonic stem cell, Patski, stimulated activated CD8-positive, alpha-beta memory T cell, naive thymus-derived CD8-positive, alpha-beta T cell, naive thymus-derived CD4-positive, alpha-beta T cell, stimulated activated naive CD8-positive, alpha-beta T cell, stimulated activated CD8-positive, alpha-beta T cell, stimulated activated CD4-positive, alpha-beta memory T cell, stimulated activated CD4-positive, alpha-beta T cell, stimulated activated naive CD4-positive, alpha-beta T cell, IMR-90, Caco-2, A673, PC-9, PC-3, Panc1, Calu3, OCI-LY7, T-cell, activated T-cell
characterized to standards with exemption
Mus musculus
embryonic stem cell, Patski
characterized to standards with exemption
- Status
- released
- Source (vendor)
- BioLegend
- Product ID
- 664912
- Lot ID
- B233183
- Characterized targets
- POLR2A (Homo sapiens), POLR2A (Mus musculus)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG2a
- Antigen description
- Clone 8WG16 recognizes YSPTSPS in the C-terminal domain which is conserved in wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.
- Aliases
- yijun-ruan:pol2_antibody
- External resources
Characterizations
POLR2A (Mus musculus)
embryonic stem cellPatski
Method: immunoblot
compliant
- Caption
- Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel 01.18.2022.
- Submitter comment
- This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
- Reviewer comment
- Approved by Erin Mendenhall 01.18.2022.
- Submitted by
- Khine Lin
- Lab
- Charles Lee
- Grant
- 3UM1HG009409-04S1
POLR2A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
exempt from standards
- Caption
- Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
- Submitter comment
- This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
- Reviewer comment
- Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
- Submitted by
- Idan Gabdank
- Lab
- Yijun Ruan, JAX
- Grant
- UM1HG009409
- Download
- exempted.png
POLR2A (Homo sapiens)
K562CD4-positive naive resting alpha-beta T cellactivated CD8-positive, alpha-beta T cellCD8-positive naive resting alpha-beta T cellGM12878activated naive CD4-positive, alpha-beta T cellactivated CD4-positive, alpha-beta memory T cellactivated CD4-positive, alpha-beta T cellactivated naive CD8-positive, alpha-beta T cellactivated CD8-positive, alpha-beta memory T cellCD8-positive, alpha-beta memory T cellMCF-7activated B cellHepG2RWPE1B cellCD8-positive, alpha-beta T cellCD4-positive, alpha-beta memory T cellAG04449HEK293BJAG04450CD4-positive, alpha-beta T cellcommon myeloid progenitor, CD34-positiveSK-N-SHGM10248MCF 10AHEK293TGM12878A549endothelial cell of umbilical veinHCT116WTC11embryonic stem cellPatskistimulated activated CD8-positive, alpha-beta memory T cellnaive thymus-derived CD8-positive, alpha-beta T cellnaive thymus-derived CD4-positive, alpha-beta T cellactivated CD4-positive, alpha-beta T cellstimulated activated naive CD8-positive, alpha-beta T cellstimulated activated CD8-positive, alpha-beta T cellCD8-positive, alpha-beta memory T cellstimulated activated CD4-positive, alpha-beta memory T cellstimulated activated CD4-positive, alpha-beta T cellstimulated activated naive CD4-positive, alpha-beta T cellIMR-90Caco-2A673PC-9PC-3Panc1Calu3OCI-LY7T-cellactivated T-cell
Method: immunoblot
exempt from standards
- Caption
- Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types and tissues are exempted for this antibody by the ENCODE antibody review panel because it is a well-characterized monoclonal antibody (clone 8WG16) that has been in use without issue by the community for many years.
- Submitter comment
- This is a well characterized antibody already used in many ENCODE experiments. It is also previously known as Convance cat# MMS-126R: http://www.biolegend.com/purified-anti-rna-polymerase-ii-rpb1-antibody-11666.html (clone 8WG16)
- Reviewer comment
- This is a well characterized antibody already used in many ENCODE experiments.
- Submitted by
- Idan Gabdank
- Lab
- Yijun Ruan, JAX
- Grant
- UM1HG009409
- Download
- exempted.png