ENCAB778YPQ
Antibody against Homo sapiens DISC1
Homo sapiens
at least one cell type or tissue
not pursued
Homo sapiens
K562
not characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- 710203
- Lot ID
- unknown
- Characterized targets
- DISC1 (Homo sapiens)
- Host
- rabbit
- Purification
- Protein A
- Isotype
- IgG
- Antigen description
- Peptide corresponding to amino acids 419–428 of human disrupted in schizophrenia 1
- External resources
Characterizations
DISC1 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using DISC1 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal DISC1 Antibody (4HCLC). This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Invitrogen_710203_unknown_DISC1.png
DISC1 (Homo sapiens)
K562
Method: immunoprecipitation
not compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using DISC1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DISC1 antibody (lanes under 'DISC1').
- Submitter comment
- big band at 220 kDa that makes up more than 50% of the lane signal.
- Reviewer comment
- big band at 220 kDa that makes up more than 50% of the lane signal.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005