1. Antibodies
  2. Homo sapiens + Mus musculus
  3. HIST2H3A + HIST2H3D + HIST2H3C + Hist1h3d + Hist1h3b + Hist2h3c2 + Hist2h3c1 + Hist1h3e + Hist1h3f + Hist2h3b + Hist1h3c

ENCAB813FEB

Antibody against Homo sapiens H3K4me2, Mus musculus H3K4me2

Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
CS200547
Lot ID
NG1859589
Host
mouse
Isotype
IgG1κ
Antigen description
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, dimethylated on Lys4, conjugated to KLH
Aliases
bing-ren:26.3
External resources

Characterizations

H3K4me2 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me2 peptide 96.6% (noted with red #1) and the H3K79me1 peptide 3.4% (noted with red #2).
Submitted by
Cricket Sloan
Lab
Bing Ren, UCSD
H3K4me2 (Mus musculus)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me2 peptide 96.6% (noted with red #1) and the H3K79me1 peptide 3.4% (noted with red #2).
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me2 (Mus musculus)
TT2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
An immunoblot using whole cell extract of two human cell lines and one mouse ES cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me2 (Homo sapiens)
HCT116HEK293
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
An immunoblot using whole cell extract of two human cell lines and one mouse ES cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD