ENCAB848AYT
Antibody against Homo sapiens CELF1
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN034PW
- Lot ID
- 001
- Characterized targets
- CELF1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, N-terminus of human CUGBP1
- External resources
Characterizations
CELF1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against CUGBP1 in K562 whole cell lysate using CUGBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown CUGBP1. CUGBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- CUGBP1-K562_Secondary_Western.png
CELF1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against CUGBP1 in HepG2 whole cell lysate using CUGBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against CUGBP1. CUGBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- CUGBP1_Secondary_Western.png
CELF1 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of MCF7 whole cell lysate using CELF1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-CUGBP1(Human)mAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN034PW_001_CELF1.png
CELF1 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB characterization of CELF1 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-CUGBP1(Human)mAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN034PW_001_CELF1_K562.png