1. Antibodies
  2. Homo sapiens
  3. E4F1

ENCAB944PGR

Antibody against Homo sapiens E4F1

Homo sapiens
K562, GM12878, MCF-7, HEK293T
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-832A
Lot ID
3
Characterized targets
E4F1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-812
External resources

Characterizations

E4F1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A300-832A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 83.496
Submitter comment
The greater band size is due to capturing a complex as opposed to a single protein. Uniprot: [Q66K89] states that E4F1 forms a complex with CDKN2A and p53. Three publications report the identification of interaction of E4F1 with p53. The size of p53 is 42KDa.
Reviewer comment
Not within 20% of expected size
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E4F1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-832A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 83.496
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E4F1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-832A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Marked band also in IgG
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E4F1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A300-832A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 83.496.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 E4F1.JPG
E4F1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody A300-832A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 83.496.
Submitter comment
The greater band size is due to capturing a complex as opposed to a single protein. Uniprot: [Q66K89] states that E4F1 forms a complex with CDKN2A and p53. Three publications report the identification of interaction of E4F1 with p53. The size of p53 is 42KDa.
Reviewer comment
Not within 20% of expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
E4F1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A300-832A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 83.496
Submitter comment
The greater band size is due to capturing a complex as opposed to a single protein. Uniprot: [Q66K89] states that E4F1 forms a complex with CDKN2A and p53. Three publications report the identification of interaction of E4F1 with p53. The size of p53 is 42KDa.
Reviewer comment
Not within 20% of expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
E4F1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A300-832A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 83.496
Submitter comment
The greater band size is due to capturing a complex as opposed to a single protein. Uniprot: [Q66K89] states that E4F1 forms a complex with CDKN2A and p53. Three publications report the identification of interaction of E4F1 with p53. The size of p53 is 42KDa.
Reviewer comment
Not within 20% of expected size
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford