ENCAB961BDT
Antibody against Homo sapiens LEF1
Homo sapiens
K562, HEK293T
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-486A
- Lot ID
- 1
- Characterized targets
- LEF1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1038
- External resources
Characterizations
LEF1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-486A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Molecular weight: 44201 Da
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1.jpg
LEF1 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target LEF1 is represented by the attached position weight matrix (PWM), and is derived from ENCFF003ZTL. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.664827415607 Global enrichment Z-score: 3.14420220051 Positional bias Z-score: 6.14922696075 Peak rank bias Z-score: 2.84354803141 Enrichment rank: 2.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
LEF1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A303-486A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 44.201
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt1115_4-LEF1-A303-486A.JPG