ENCBS038REP / cell line
Summary
- Status
- released
- Term name
- SK-N-SH
- Term ID
- EFO:0003072
- Summary
- Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens GLIS3 treated with 6 μM all-trans-retinoic acid for 48 hours
- Culture harvest date
- 2018-09-26
- Separated from biosample
- ENCBS651SCV
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-11121, michael-snyder:pB-eGFP-GLIS3 SK-N-SH rep8
Treatment details
- Treatment
- 6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
- Type
- chemical
- Purpose
- activation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM708KYJ | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000ABD
- Aliases
- encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
- Species
- Homo sapiens
- Life stage
- child
- Age
- 4 years
- Sex
- female
- Health status
- Metastatic neuroblastoma from bone marrow
- External resources
- References
Documents
immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS071NZE (SK-N-SH eGFP-GLIS3 replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 112.0 kD as indicated with an arrow.
- Submitted by
- Jessica Au
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Submitter comment
- DCC submitted redundant biosample characterization based off of this biosample-characterizations/536f2fd5-e6e5-4721-9ee0-f5ebdc5d3cba/
- Documents
growth protocol
Description: Cell growth protocol for SK-N-SH cells (version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
transfection protocol
Description excerpt: SK-N-SH transfection protocol - Version 1 (Replicates split at T-75 stage, 200…
- Description
- SK-N-SH transfection protocol - Version 1 (Replicates split at T-75 stage, 200 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
treatment protocol
Description: RA treatment protocol for SK-N-SH cells (version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996