ENCBS111IHC / cell line
Summary
- Status
- released
- Term name
- HepG2
- Term ID
- EFO:0001187
- Summary
- Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SRSF7
- Description
- RNA-seq on HepG2 cells treated with a CRISPR gRNA against SRSF7. (SRSF7_BGHcLV03-5)
- Product ID
- HB-8065
- Lot ID
- 59635738
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Xintao Wei
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:SRSF7_BGHcLV03-5
- Tags
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM146LLT | deletion | repression | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAC
- Aliases
- encode:donor of HepG2, bradley-bernstein:Donor of HepG2
- Species
- Homo sapiens
- Life stage
- child
- Age
- 15 years
- Sex
- male
- Health status
- hepatocellular carcinoma
- Ethnicity
- European
- External resources
- References
Documents
growth protocol
Description: Growth protocol for HepG2
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
general protocol
Description excerpt: Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation…
- Description
- Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation and Characterization Document
- Submitted by
- Zachary Myers
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005