ENCBS159HRH / cell line
Summary
- Status
- released
- Term name
- C4-2B
- Term ID
- EFO:0006365
- Summary
- Homo sapiens C4-2B cell line expressing RNAi targeting H. sapiens ZFX
- Description
- C4-2B cells treated with a siRNA knockdown against ZFX, used in RNA-seq experiments.
Attribution
ENCODE3 project
- Lab
- Peggy Farnham, USC
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Heather Witt
- Source
- ViroMed Laboratories
- Project
- ENCODE
- External resources
- Aliases
- peggy-farnham:B-C4-2B-ZFX-rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM989SXT | interference | repression | RNAi |
|
Donor information
- Status
- released
- Accession
- ENCDO000ACX
- Aliases
- encode:donor of C4-2B, encode:donor of LNCaP clone FGC, encode:donor of LNCAP
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 50 years
- Sex
- male
- Health status
- prostate adenocarcinoma
- Ethnicity
- European
- External resources
- References
Documents
transfection protocol
Description: Basic transfection protoco with Dharmafectl from Dharmacon
- Submitted by
- Heather Witt
- Lab
- Peggy Farnham, USC
- Grant
- U54HG006996
other
Description: Basic siRNA suspension protocol from Dharmacon
- Submitted by
- Heather Witt
- Lab
- Peggy Farnham, USC
- Grant
- U54HG006996