ENCBS168VCZ / cell line

Summary

Status: released
Term name: HEK293
Term ID: EFO:0001182
Summary: Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens ZNF76
Description: HEK293 cell line stably expressing N-terminal tagged eGFP-ZNF76 under the control of a CMV promoter.
Originated from biosample: ENCBS749QJL

Attribution

Lab: Michael Snyder, Stanford
Award PI: Michael Snyder, Stanford
Submitted by: Tejaswini Mishra
Source: Timothy Hughes
Project: ENCODE
External resources: GEO:SAMN06121653
Cellosaurus:CVCL_0045
Aliases: michael-snyder:HEK_eGFP-ZNF76_FRT_ChIP_B2

Genetic modifications

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM715FKW	insertion	tagging	site-specific recombination		Target ZNF76-human Integration site random

Donor information

Status: released
Accession: ENCDO000ABF
Aliases: encode:donor of HEK293, encode:donor of HEK293T
Species: Homo sapiens
Life stage: embryonic
Age: Unknown
Sex: female
Health status: unknown
External resources: GEO:SAMN06122596
References: doi:10.1096/fj.01-0995fje
PMID:11967234

Documents

Description: Stable cell line generation with Thermo Fisher's Flp-In system.

Attachment from submitter

Attachment from submitters

Thermo_Fisher_Flp-In_system.png

Submitted by: Tejaswini Mishra
Lab: Michael Snyder, Stanford
Grant: U54HG006996