ENCBS317OTM / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZKSCAN3
- Separated from biosample
- ENCBS342YQY
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-11084, michael-snyder:pB-eGFP-ZKSCAN3 K562 rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM332FTE | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS317OTM (K562 eGFP-ZKSCAN3 replicate 1) cells using anti-eGFP antibody.The image shows western blot analysis of immunoprecipitation. Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).The target molecular weight is indicated.
- Submitted by
- Tao Wang
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
growth protocol
Description: Cell growth protocol for K562 cells (version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
transfection protocol
Description excerpt: K562 transfection protocol - Version 2 (Replicates split right after…
- Description
- K562 transfection protocol - Version 2 (Replicates split right after transfection, 50 and 500 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442