ENCBS349GQD / cell line
Summary
- Status
- released
- Term name
- HEK293
- Term ID
- EFO:0001182
- Summary
- Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens ZNF32
- Description
- HEK293 cell line stably expressing N-terminal tagged eGFP-ZNF32 under the control of a CMV promoter.
- Originated from biosample
- ENCBS749QJL
Attribution
ENCODE3 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Tejaswini Mishra
- Source
- Timothy Hughes
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:HEK_eGFP-ZNF32_FRT_ChIP_B1
- Submitter comment
- WARNING: The tagged TF could not be verified by AP-MS and may not reflect the annotated zinc finger target in the metadata.
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM997QWN | insertion | tagging | site-specific recombination |
|
Donor information
- Status
- released
- Accession
- ENCDO000ABF
- Aliases
- encode:donor of HEK293, encode:donor of HEK293T
- Species
- Homo sapiens
- Life stage
- embryonic
- Age
- Unknown
- Sex
- female
- Health status
- unknown
- External resources
- References
Documents
transfection protocol
Description: Stable cell line generation with Thermo Fisher's Flp-In system.
- Submitted by
- Tejaswini Mishra
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996