ENCBS372QTG / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mls-2
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to MLS-2
- Life stage
- mixed stage (embryonic)
- Age
- Unknown
- Synchronization timepoint
- egg bleaching + 2.0 hours
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:MLS-2_GFP_OP645_EM_Rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM510LWV | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO226NKQ
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- unc-119(tm4063) III; wgIs645
- Sex
- mixed
- Strain name
- OP645
- External resources
Documents
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355