ENCBS559ENC / cell line
Summary
- Status
- released
- Term name
- GM03348
- Term ID
- CLO:0016536
- Summary
- Homo sapiens GM03348 cell line genetically modified (insertion) using transduction targeting H. sapiens MYOD1
- Description
- skin fibroblast; GM03348 skin fibroblasts (Coriell) were transduced with an induceable lentivirus (pAK111-TRE-3xflag hMyoD-T2A-dsRed) containing get inducible MyoD. Cells were selected in 1ug per ml puro (Sigma catalog P8833) to obtain a pure cell population. Cells were cultured for 10 days (NO DOXYCYCLINE)
- Product ID
- GM03348
Attribution
ENCODE2 project
- Lab
- Gregory Crawford, Duke
- Award PI
- Gregory Crawford, Duke
- Submitted by
- Terry Furey
- Source
- Coriell
- Project
- ENCODE
- External resources
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM883SVN | insertion | tagging | transduction |
|
Donor information
- Status
- released
- Accession
- ENCDO186AAA
- Aliases
- encode:donor_of_Fibrobl_GM03348
- Species
- Homo sapiens
- Life stage
- child
- Age
- 10 years
- Sex
- male
- Health status
- apparently healthy
- Ethnicity
- European
Documents
growth protocol
Description: GM03348 Fibroblast culture conditions (Coriell)
- Submitted by
- Gregory Crawford
- Lab
- Gregory Crawford, Duke
- Grant
- U54HG004563