ENCBS618ENC / cell line
Summary
- Status
- released
- Term name
- MCF 10A
- Term ID
- EFO:0001200
- Summary
- Homo sapiens MCF 10A cell line stably expressing SRC treated with 0.01% ethanol for 36 hours
- Description
- mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
- Product ID
- MCF10A-Er-Src
- Lot ID
- NULL
Attribution
ENCODE2 project
- Lab
- Kevin Struhl, HMS
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Kevin Struhl
- Source
- Kevin Struhl
- Project
- ENCODE
- External resources
Treatment details
- Treatment
- 0.01 percent ethanol (CHEBI:16236) for 36 hours
- Type
- chemical
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM980FIG | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAP
- Aliases
- encode:donor of MCF10A-Er-Src, encode:donor of MCF 10A
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 36 years
- Sex
- female
- Health status
- fibrocystic disease
- Ethnicity
- European
- External resources
- References
Documents
growth protocol
Description: MCF10A-Er-Src
- Submitted by
- Kevin Struhl
- Lab
- Kevin Struhl, HMS
- Grant
- U54HG004558
treatment protocol
Description: Transformation conditions of MCF10A-Er-Src
- Submitted by
- Kevin Struhl
- Lab
- Kevin Struhl, HMS
- Grant
- U54HG004558