ENCBS673GRR / cell line

Status: released
Term name: K562
Term ID: EFO:0002067
Summary: Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF764
Culture harvest date: 2017-06-06
Separated from biosample: ENCBS993QKW

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM650SRE	insertion	tagging	CRISPR		Target ZNF764-human

Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…

Caption: Immunoprecipitation was performed on nuclear extracts from biosample ENCBS016DSZ (K562 eGFP-ZNF764 replicate 1) cells using anti-eGFP antibody.The image shows western blot analysis of immunoprecipitation. Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).The target molecular weight is indicated.
Submitted by: Jessica Au
Lab: Michael Snyder, Stanford
Grant: UM1HG009442
Submitter comment: DCC submitted redundant biosample characterization based off of this biosample-characterizations/45697c2e-7879-4c4f-87af-80ba0d2c0816/ object

Description: Cell growth protocol for K562 cells (version 1).

Description excerpt: K562 transfection protocol - Version 1 (Replicates split at T-75 stage, 50 and…

Description: K562 transfection protocol - Version 1 (Replicates split at T-75 stage, 50 and 500 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442