1. Biosamples
  2. cell line
  3. Homo sapiens
  4. K562

ENCBS759FXB / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF830
Passage number
5
Separated from biosample
ENCBS803UTU

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Tao Wang
Source
ATCC
Project
ENCODE
Aliases
michael-snyder:pB-11685, michael-snyder:pB-eGFP-ZNF830 K562 Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM849HFRinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Attachment from submitters
12-4-2018 K562 CRISPR IP.jpg
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS759FXB (K562 eGFP-ZNF830 replicate 2) cells using anti-eGFP antibody.The image shows western blot analysis of immunoprecipitation. Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).The target molecular weight is indicated.
Submitted by
Tao Wang
Lab
Michael Snyder, Stanford