ENCBS959EEP / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZBTB1
- Separated from biosample
- ENCBS650NJZ
- Parent of biosamples
- ENCBS954DUF, ENCBS870OFG, ENCBS058RAN
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-11111, michael-snyder:pB-eGFP-ZBTB1 K562
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM091KNO | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
growth protocol
Description: Cell growth protocol for K562 cells (version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
transfection protocol
Description excerpt: K562 transfection protocol - Version 2 (Replicates split right after…
- Description
- K562 transfection protocol - Version 2 (Replicates split right after transfection, 50 and 500 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442