ENCBS963ISJ / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens KDM2B
- Separated from biosample
- ENCBS557KCI
- Parent of biosamples
- ENCBS003XEJ, ENCBS753XIM, ENCBS593WGG, ENCBS134AFA
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-10148, michael-snyder:pB-eGFP-KDM2B K562
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM610WPQ | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
transfection protocol
- Description
- K562 transfection protocol - Version 2 (Replicates split right after transfection, 50 and 500 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442