Experiment summary for ENCSR027CZW
doi:10.17989/ENCSR027CZW
Summary
- Status
- released
- Assay
- ChIP-seq (Control ChIP-seq)
- Control type
- input library
- Biosample summary
- Caenorhabditis elegans strain RW12173 (st12173[egl-44::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) whole organism young adult (66.5 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using CRISPR targeting C. elegans egl-44
- Biosample Type
- whole organisms
- Replication type
- unreplicated
- Description
- This is the ChIP-seq control of transgenic worms expressing egl-44-eGFP fusion proteins.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina NovaSeq 6000
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:egl-44_RW12173_youngadult_1_INPUT
- Date released
- December 19, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain RW12173 (st12173[egl-44::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) young adult (66.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans egl-44 | ENCBS762IVB | ENCGM889RIE | ENCAB728YTO | ENCLB573ODA |