Experiment summary for ENCSR117TRB
doi:10.17989/ENCSR117TRB
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- lin-54
- Biosample summary
- Caenorhabditis elegans strain OP743 (unc-119(tm4063); wgIs743 [lin-54::TY1::EGFP::3xFLAG(Fosmid: lin-54) + unc-119(+)]) whole organism young adult (65 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans lin-54
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing lin-54-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:LIN-54_OP743_yAd
- Date submitted
- February 26, 2018
- Date released
- March 1, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP743 (unc-119(tm4063); wgIs743 [lin-54::TY1::EGFP::3xFLAG(Fosmid: lin-54) + unc-119(+)]) mixed sex young adult (65 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lin-54 | ENCBS628IZZ | ENCGM568NMR | ENCAB728YTO | ENCLB122OYU |
| 1 | 1 | Caenorhabditis elegans strain OP743 (unc-119(tm4063); wgIs743 [lin-54::TY1::EGFP::3xFLAG(Fosmid: lin-54) + unc-119(+)]) mixed sex young adult (65 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lin-54 | ENCBS915NTC | ENCGM568NMR | ENCAB728YTO | ENCLB911ZRS |