Experiment summary for ENCSR133RTP

doi:10.17989/ENCSR133RTP

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
madf-10
Biosample summary
Caenorhabditis elegans strain OP508 (unc-119(tm4063) III; wgIs508) whole organism hermaphrodite young adult 68 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans madf-10
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing MADF-10-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:MADF-10-OP508-yAd
Date submitted
July 29, 2015
Date released
May 10, 2016
Submitter comment
ENCFF258RVD, ENCFF062WAG, ENCFF822VGF, ENCFF717DLE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP508 (unc-119(tm4063) III; wgIs508) hermaphrodite young adult 68 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans madf-10ENCBS064BDEENCGM161MFYENCAB728YTOENCLB216KDQ
11Caenorhabditis elegans strain OP508 (unc-119(tm4063) III; wgIs508) hermaphrodite young adult 68 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans madf-10ENCBS672SLNENCGM161MFYENCAB728YTOENCLB517JHT