Experiment summary for ENCSR146KCR
doi:10.17989/ENCSR146KCR
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- ztf-1
- Biosample summary
- Caenorhabditis elegans strain OP207 (wgIs207(WRM0628B_D10(pRedFlp-Hgr)::unc-119-Nat(ztf-1[24953]::2XTY1-eGFP(C.ele)-3XFLAG)dFRT )) whole organism L2 larva (28.5 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans ztf-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing ztf-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:ztf-1_OP207_L2larva_1_IP
- Date released
- November 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP207 (wgIs207(WRM0628B_D10(pRedFlp-Hgr)::unc-119-Nat(ztf-1[24953]::2XTY1-eGFP(C.ele)-3XFLAG)dFRT )) L2 larva (28.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ztf-1 | ENCBS115AUN | ENCGM016DVJ | ENCAB728YTO | ENCLB480RMX |
| 2 | 1 | Caenorhabditis elegans strain OP207 (wgIs207(WRM0628B_D10(pRedFlp-Hgr)::unc-119-Nat(ztf-1[24953]::2XTY1-eGFP(C.ele)-3XFLAG)dFRT )) L2 larva (28.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ztf-1 | ENCBS461QQS | ENCGM016DVJ | ENCAB728YTO | ENCLB730NSF |