Experiment summary for ENCSR162MNX
doi:10.17989/ENCSR162MNX
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- tbx-2
- Biosample summary
- Caenorhabditis elegans strain OP159 (unc-119(ed3) III; wgIs159) whole organism hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans tbx-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing TBX-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:TBX-2-OP159-LE
- Date submitted
- July 30, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF367SPO, ENCFF808INB, ENCFF912KQG, ENCFF503OVR were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP159 (unc-119(ed3) III; wgIs159) hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tbx-2 | ENCBS685HJB | ENCGM443NQB | ENCAB728YTO | ENCLB832QGN |
| 2 | 1 | Caenorhabditis elegans strain OP159 (unc-119(ed3) III; wgIs159) hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tbx-2 | ENCBS059THY | ENCGM443NQB | ENCAB728YTO | ENCLB192TSX |