Experiment summary for ENCSR279HMU
doi:10.17989/ENCSR279HMU
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens HepG2 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in HepG2 cells followed by RNA-seq. (non-target-BGHLV22)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.9 [2-1]9.8 [1-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:non-target-BGHLV22
- Date submitted
- August 13, 2015
- Date released
- August 18, 2015
- Gene Silencing Series
- ENCSR371FFJ , ENCSR153LWS , ENCSR840NRJ , ENCSR857JCZ , ENCSR418EER , ENCSR494NLK , ENCSR879UEK , ENCSR078ZFS , ENCSR974NRI , ENCSR506CST , ENCSR812YDZ , ENCSR787JDA , ENCSR405LHE , ENCSR373VUD , ENCSR491IUN , ENCSR317QGL , ENCSR069UQV
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS472BZM | ENCGM266AWP | ENCLB037LAW |
| 1 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS063KED | ENCGM266AWP | ENCLB006OEV |