Experiment summary for ENCSR307OHT
doi:10.17989/ENCSR307OHT
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- egl-44
- Biosample summary
- Caenorhabditis elegans strain RW12173 (st12173[egl-44::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) whole organism young adult (66.5 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using CRISPR targeting C. elegans egl-44
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing egl-44-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:egl-44_RW12173_youngadult_1_IP
- Date released
- November 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain RW12173 (st12173[egl-44::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) young adult (66.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans egl-44 | ENCBS416LHQ | ENCGM889RIE | ENCAB728YTO | ENCLB467LEN |
| 2 | 1 | Caenorhabditis elegans strain RW12173 (st12173[egl-44::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) young adult (66.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans egl-44 | ENCBS023CGY | ENCGM889RIE | ENCAB728YTO | ENCLB078CDO |