Experiment summary for ENCSR320MVY
doi:10.17989/ENCSR320MVY
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- snu-23
- Biosample summary
- Caenorhabditis elegans strain OP528 (wgIs528(snu-23::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0094_4B11);unc-119(+));unc-119 (tm4063)) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans snu-23
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing SNU-23-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:SNU-23-OP528-L1
- Date submitted
- July 29, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF021IIR, ENCFF673YEB, ENCFF323BXM, ENCFF026VXC were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP528 (wgIs528(snu-23::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0094_4B11);unc-119(+));unc-119 (tm4063)) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans snu-23 | ENCBS346GXU | ENCGM601RHK | ENCAB728YTO | ENCLB031MPL |
| 2 | 1 | Caenorhabditis elegans strain OP528 (wgIs528(snu-23::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0094_4B11);unc-119(+));unc-119 (tm4063)) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans snu-23 | ENCBS643HGH | ENCGM601RHK | ENCAB728YTO | ENCLB993UNP |