1. Experiments
  2. ChIP-seq
  3. Caenorhabditis elegans
  4. whole organism

Experiment summary for ENCSR345JIR

doi:10.17989/ENCSR345JIR

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
sox-4
Biosample summary
Caenorhabditis elegans strain OP517 (unc-119(tm4063) III; wgIs517) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans sox-4
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing SOX-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:SOX-4-OP517-L1
Date submitted
July 29, 2015
Date released
May 24, 2016
Submitter comment
ENCFF127QCN, ENCFF864TUY, ENCFF741UBD, ENCFF612NJJ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP517 (unc-119(tm4063) III; wgIs517) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans sox-4ENCBS069FMCENCGM544OTRENCAB728YTOENCLB544OGT
11Caenorhabditis elegans strain OP517 (unc-119(tm4063) III; wgIs517) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans sox-4ENCBS584WVEENCGM544OTRENCAB728YTOENCLB255ZPB

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