Experiment summary for ENCSR442XYW
doi:10.17989/ENCSR442XYW
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- madf-2
- Biosample summary
- Caenorhabditis elegans strain OP587 (unc-119(tm4063) III; wgIs587) whole organism late embryo 3 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans madf-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing MADF-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:MADF-2-OP587-LE
- Date submitted
- October 5, 2017
- Date released
- November 6, 2017
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
2 | 1 | Caenorhabditis elegans strain OP587 (unc-119(tm4063) III; wgIs587) mixed sex late embryo 3 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans madf-2 | ENCBS655ZDX | ENCGM890CXI | ENCAB728YTO | ENCLB917VJO |
1 | 1 | Caenorhabditis elegans strain OP587 (unc-119(tm4063) III; wgIs587) mixed sex late embryo 3 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans madf-2 | ENCBS233BWE | ENCGM890CXI | ENCAB728YTO | ENCLB074CMY |