Experiment summary for ENCSR481UIZ
doi:10.17989/ENCSR481UIZ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- ztf-29
- Biosample summary
- Caenorhabditis elegans strain RW12283 (st12283[ztf-29 ::TY1::EGFP::3xFLAG] III) whole organism midembryo (0 hours) using egg bleaching genetically modified (insertion) using CRISPR targeting C. elegans ztf-29
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing ztf-29-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:ztf-29_RW12283_midembryonic_1_IP
- Date released
- November 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain RW12283 (st12283[ztf-29 ::TY1::EGFP::3xFLAG] III) midembryo (0 hours) using egg bleaching whole organism genetically modified (insertion) using CRISPR targeting C. elegans ztf-29 | ENCBS455DJM | ENCGM847NGF | ENCAB728YTO | ENCLB324GYU |
| 2 | 1 | Caenorhabditis elegans strain RW12283 (st12283[ztf-29 ::TY1::EGFP::3xFLAG] III) midembryo (0 hours) using egg bleaching whole organism genetically modified (insertion) using CRISPR targeting C. elegans ztf-29 | ENCBS251OXT | ENCGM847NGF | ENCAB728YTO | ENCLB644UDH |