1. Experiments
  2. ChIP-seq
  3. Caenorhabditis elegans
  4. whole organism

Experiment summary for ENCSR557ZYT

doi:10.17989/ENCSR557ZYT

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
nhr-80
Biosample summary
Caenorhabditis elegans strain OP460 (unc-119(tm4063) III; wgIs460) whole organism hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans nhr-80
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing NHR-80-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:NHR-80-OP460-yAd
Date submitted
July 29, 2015
Date released
May 10, 2016
Submitter comment
ENCFF981KJJ, ENCFF495FEW, ENCFF811NRK, ENCFF586LVZ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP460 (unc-119(tm4063) III; wgIs460) hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans nhr-80ENCBS547GQHENCGM573LQIENCAB728YTOENCLB031SQX
21Caenorhabditis elegans strain OP460 (unc-119(tm4063) III; wgIs460) hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans nhr-80ENCBS044GYHENCGM573LQIENCAB728YTOENCLB535GUJ

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