1. Experiments
  2. single-cell RNA sequencing assay
  3. Mus musculus
  4. Purkinje cell

Experiment summary for ENCSR620HJQ

doi:10.17989/ENCSR620HJQ

Summary

Status
archived
Assay
single-cell RNA sequencing assay (scRNA-seq)
Control type
control
Biosample summary
Mus musculus strain B6CASTF1/J Purkinje cell male adult (14 months) originated from cerebellum Purkinje cell layer
Biosample Type
primary cell
Replication type
unreplicated
Description
RNA-seq on single cell equivalents of 13 excised and homogenized Purkinje cells from a 14 month old male hybrid Castaneus X C57Bl6 mouse
Nucleic acid type
RNA
Library starting quantity
1cell-equivalent
Size range
>200
Lysis method
Clontech UltraLow for Illumina sequencing
Size selection method
SPRI beads
Strand specificity
unstranded
Spike-ins datasets
ENCSR013YHQ, ENCSR133ALU

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Date submitted
May 29, 2015
Date released
October 13, 2015
Submitter comment
Estimates of technical measurement error from low input mass RNA-seq libraries.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
12Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS525QEPENCLB243CVS
112Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS268XKFENCLB318YQB
13Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS251NNJENCLB204EWE
110Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS007DRRENCLB461BIJ
113Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS187KIVENCLB407NSA
111Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS313XXNENCLB580JUS
11Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS297ITFENCLB714KUY
15Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS387FIKENCLB729FQY
16Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS912HHGENCLB988OPM
14Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS194DCOENCLB751POF
18Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS689OWRENCLB981WTU
19Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell originated from cerebellum Purkinje cell layerENCBS980LDZENCLB364NZB

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