Experiment summary for ENCSR643BKM

doi:10.17989/ENCSR643BKM

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Biosample summary
Caenorhabditis elegans strain OP615 (unc-119(tm4063) III; wgIs615) whole organism late embryo 4.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans Y22D7AL.16
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing Y22D7AL.16-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Y22D7AL.16-OP615-LE
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF615LPW, ENCFF326USC, ENCFF657OXS, ENCFF260MWA were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP615 (unc-119(tm4063) III; wgIs615) mixed sex late embryo 4.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans Y22D7AL.16ENCBS207AFWENCGM583ZMSENCAB728YTOENCLB321QUZ
11Caenorhabditis elegans strain OP615 (unc-119(tm4063) III; wgIs615) mixed sex late embryo 4.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans Y22D7AL.16ENCBS356TCCENCGM583ZMSENCAB728YTOENCLB998ERT