Experiment summary for ENCSR668BTV
doi:10.17989/ENCSR668BTV
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- mls-2
- Biosample summary
- Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) whole organism mixed stage (embryo) 2 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans mls-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing MLS-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:MLS-2-OP645-EM
- Date submitted
- October 4, 2016
- Date released
- October 10, 2016
- Submitter comment
- ENCFF328QIP, ENCFF251ZRR, ENCFF595KCQ, ENCFF088XAX were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mls-2 | ENCBS797YYO | ENCGM510LWV | ENCAB728YTO | ENCLB478OSH |
| 1 | 1 | Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mls-2 | ENCBS372QTG | ENCGM510LWV | ENCAB728YTO | ENCLB976NIS |