Experiment summary for ENCSR837VHE
doi:10.17989/ENCSR837VHE
Summary
- Status
- released
- Assay
- DNase-seq
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 2 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- DNase-seq on A549 cell line treated with 100 nM dexamethasone for 2 hours.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 2 hours
- Nucleic acid type
- DNA
- Library starting quantity
- 220ng
- Size selection method
- gel
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:t2_dnase_seq
- Date submitted
- March 2, 2016
- Date released
- May 5, 2016
- Treatment Time Series
- ENCSR482QFS
- Annotation (gkmsvm-model)
- Submitter comment
- DNase-seq libraries were sequenced with four barcodes per lane to generate 50 bp single-end reads, of which only the first 20 bp were genomic DNA due to MmeI digestion (see Song and Crawford 2010).
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 2 hours | ENCBS549TVN | ENCLB326FVP |
| 2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 2 hours | ENCBS191VYO | ENCLB613CCB |
| 4 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 2 hours | ENCBS157WWD | ENCLB033FLH |
| 5 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 2 hours | ENCBS570PSF | ENCLB546RRH |